Anthurium sp. is an imported ornamental plant with high economic value in Vietnam. Consumer preferences and demand depend greatly on the colour of the spathes, so understanding the genetic diversity of varieties is necessary to create novel flowers. In this work, we aimed to characterise genetic diversity among Anthurium accessions collected from four provinces in Vietnam (Ha Noi, Thua Thien Hue, Lam Dong and Can Tho). DNA of eighteen Anthurium accessions was extracted using the cetyl trimethyl ammonium bromide (CTAB) method. Then, genomic DNA was amplified with matK and rbcL primers in PCR and sequencing to determine sequence parameters using DNAsp v6 and MEGA11 software. Three phylogenetic trees including the Neighborjoining (NJ) tree, Maximum likelihood (ML) tree and Bayesian inference (BI) tree were created to separate Anthurium accessions. In addition, ten morphological traits were also described and were cluster-analysed (UPGMA) using MVSP v3.22 software.