Conversion of an RAPD Marker to Co-dominant SCARMarker and Its Application in Marker-assisted Selectionfor Fusarium Crown and Root Rot (Frl) Resistance in Tomato
Nội dung tóm tắt: Fusarium crown and root rot of tomato (Solanum lycopersicum) is the disease caused by the fungal pathogen Fusarium oxysporum f. sp. radicis-lycopersici (FORL). The most effective way to control this disease is to plant varieties resistant to Fusarium crown and root rot. Marker tightly linked to Frl could be assisted in breeding program to introgress crown rot resistance into new varieties. In this study, we converted a published RAPD marker (UBC#116) linked to Frl (Fazio et al., 1999) into codominant SCAR marker. The marker was used to genotype 96 tomato germplasm of National Institute of Horticultural and Herbal Science (NIHHS). Among germplasm, 24 lines carried resistant allele. Published RAPD (UBC#116) and converted co-dominant SCAR markers provided consistent results. Three tomato lines, which have not intense resistant fragments using UBC#116, shown heterozygous pattern using SCAR marker. Our result demonstrates the usefulness of the codominant SCAR marker for marker-assisted selection (MAS) for Fusarium crown and root rot resistance in tomato
Thể loại: Không thuộc ISI/SCOPUS, có ISSN/ISBN
Tác giả: Trương Thị Hồng Hải, HS. Choi, MC. Cho
Đơn vị: Ban lãnh đạo viện
Đăng tại: Korean Journal Horticulture Science Technology 29
Tập, (số), trang: 29, SUPPL. I, 103
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Điểm số theo QĐ của HĐ chức danh GS Nhà nước:
Năm công bố: 2011
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Số lần xem: 328
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