Conversion of an RAPD Marker to Co-dominant SCARMarker and Its Application in Marker-assisted Selectionfor Fusarium Crown and Root Rot (Frl) Resistance in Tomato
Summary: Fusarium crown and root rot of tomato (Solanum lycopersicum) is the disease caused by the fungal pathogen Fusarium oxysporum f. sp. radicis-lycopersici (FORL). The most effective way to control this disease is to plant varieties resistant to Fusarium crown and root rot. Marker tightly linked to Frl could be assisted in breeding program to introgress crown rot resistance into new varieties. In this study, we converted a published RAPD marker (UBC#116) linked to Frl (Fazio et al., 1999) into codominant SCAR marker. The marker was used to genotype 96 tomato germplasm of National Institute of Horticultural and Herbal Science (NIHHS). Among germplasm, 24 lines carried resistant allele. Published RAPD (UBC#116) and converted co-dominant SCAR markers provided consistent results. Three tomato lines, which have not intense resistant fragments using UBC#116, shown heterozygous pattern using SCAR marker. Our result demonstrates the usefulness of the codominant SCAR marker for marker-assisted selection (MAS) for Fusarium crown and root rot resistance in tomato
Type: Non-ISI/SCOPUS listed journals with ISSB/ISBN
Author: Trương Thị Hồng Hải, HS. Choi, MC. Cho
Unit: Director board of Institute
Journal:Korean Journal Horticulture Science Technology 29
Issue, Number, Pages29, SUPPL. I, 103
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Year of publication: 2011
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