Development of a co-dominant SCAR marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato (Solanum lycopersicum)
Nội dung tóm tắt: Random amplified polymorphism DNA (RAPD) and bulk segregant analysis (BSA) approaches were used to characterize the molecular marker linked to the Phytophthora infestans resistance gene Ph-3 in tomato. A total of 800 RAPD primers were screened. One RAPD marker UBC#602 was identified to be tightly linked to the Ph-3 gene. The marker was successfully converted into a co-dominant sequence characterized amplified region (SCAR) marker. The SCAR marker SCU602 was used to analyze 96 F2 progenies and fitted the expected 1:2:1 Mendelian segregation ratio. Forty one tomato inbred lines were screened using the SCAR marker in comparison with a reference marker linked to the Ph-3 gene and both markers gave the same results. SCU602 was further validated for association to resistance and its potential in MAS in 72 tomato lines and cultivars. The marker identified three genotypes harbouring the resistance allele. This SCAR marker can be used in breeding programs for the selection of the Ph-3 gene for Phytophthora infestans resistance
Thể loại: Quốc tế
Tác giả: Trương Thị Hồng Hải, Hung Ngoc Tran , Hak Soon Choi , Pue Hee Park, Hye Eun Lee
Đơn vị: Ban lãnh đạo viện
Đăng tại: European Journal of Plant Pathology
Tập, (số), trang: 136, 237–245
Số ISSN/ISBN: 0929-1873
Chỉ số Impact factor:
Điểm số theo QĐ của HĐ chức danh GS Nhà nước:
Năm công bố: 2013
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Số lần xem: 287
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