Cloning and Expression of Genes Encoding F107-C and K88-1NT Fimbrial Proteins of Enterotoxigenic Escherichia coli from Piglets

Nội dung tóm tắt: We cloned two genes coding F107-C and K88-1NT fimbrial subunits from strains E. coli C and 1NT isolated from Thua Thien Hue province, Vietnam. The mature peptide of faeG gene from strain E. coli 1NT (called faeG-1NT) is 100 % similarity with faeG gene, while the CDS of fedA gene from strain C (called fedA-C) has a similarity of 97 % with the fedA gene. Expression of the faeG-1NT and fedA-C genes in E. coli BL21 StarTM (DE3) produced proteins of *31 and 22 kDa, respectively. The effect of IPTG concentration on the K88-1NT and F107-C fimbriae production was investigated. The results showed that 0.5 mM IPTG is suitable for higher expression of K88-1NT subunit, while 0.75 mM IPTG strongly stimulated expression of F107-C subunit. The optimal induction time for expression was also examined. Generally, highest expression of K88-1NT subunit occurred after 6h of induction, while that of F107-C subunit is after 14h.

Thể loại: Không thuộc ISI/SCOPUS, có ISSN/ISBN

Tác giả: Nguyễn Hoàng Lộc, Lê Mỹ Tiểu Ngọc, Trần Thúy Lan, Hoàng Tấn Quảng, Đinh Thị Bích Lân, Phùng Thăng Long

Đơn vị: Phòng thí nghiệm Công nghệ Gen

Đăng tại: Indian Journal of Microbiology

Tập, (số), trang: 53, 4, 488-491

Số ISSN/ISBN: 0046 - 8991, E - ISSN: 0973 - 7715

Chỉ số Impact factor:

Điểm số theo QĐ của HĐ chức danh GS Nhà nước:

Năm công bố: 2013

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