Cloning and Expression of Genes Encoding F107-C and K88-1NT Fimbrial Proteins of Enterotoxigenic Escherichia coli from Piglets

 Summary: We cloned two genes coding F107-C and K88-1NT fimbrial subunits from strains E. coli C and 1NT isolated from Thua Thien Hue province, Vietnam. The mature peptide of faeG gene from strain E. coli 1NT (called faeG-1NT) is 100 % similarity with faeG gene, while the CDS of fedA gene from strain C (called fedA-C) has a similarity of 97 % with the fedA gene. Expression of the faeG-1NT and fedA-C genes in E. coli BL21 StarTM (DE3) produced proteins of *31 and 22 kDa, respectively. The effect of IPTG concentration on the K88-1NT and F107-C fimbriae production was investigated. The results showed that 0.5 mM IPTG is suitable for higher expression of K88-1NT subunit, while 0.75 mM IPTG strongly stimulated expression of F107-C subunit. The optimal induction time for expression was also examined. Generally, highest expression of K88-1NT subunit occurred after 6h of induction, while that of F107-C subunit is after 14h.

 Type: Non-ISI/SCOPUS listed journals with ISSB/ISBN

 Author: Nguyễn Hoàng Lộc, Lê Mỹ Tiểu Ngọc, Trần Thúy Lan, Hoàng Tấn Quảng, Đinh Thị Bích Lân, Phùng Thăng Long

 Unit: Laboratory of Gene technology

 Journal:Indian Journal of Microbiology

 Issue, Number, Pages53, 4, 488-491

 ISSN/ISBN:0046 - 8991, E - ISSN: 0973 - 7715

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 Year of publication: 2013

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