Genetic diversity of loach Misgurnus anguillicaudatus (Cantor, 1842) in Vietnam by randomly amplified polymorphic DNA analysis

Nội dung tóm tắt: In this study, Random Amplified Polymorphic DNA (RAPD) markers were applied to analyse the genetic diversity of samples of the Misgurnus anguillicaudatus (Cantor, 1842), collected from six localities (A Luoi, Phong Dien, Phu Vang, Phu Loc, Quang Dien, and Huong Thuy) along Thua Thien Hue in Vietnam. The six primers used in RAPD analysis amplified 91 loci, 88 (96.70%) of which were polymorphic. The percentages of polymorphic loci observed in the six populations were: 61.54% (A Luoi), 58.24% (Phong Dien), 57.14% (Phu Vang), 39.56% (Phu Loc), 63.74% (Quang Dien), and 47.25% (Huong Thuy). Data for observed and effective number of alleles, Nei's (1973) genetic diversity, and Shannon's information index, for all the six populations were 1.55, 1.30, 0.18 and 0.27, respectively. The value for total genotype diversity among populations was 0.2239 while within populations diversity was found to be 0.1769. The mean coefficient of gene differentiation value and the estimate of gene flow across the populations were found as 0.2100 and 1.8812, respectively. The Nei (1978) measures of genetic distance and identity between pairs of loach populations indicate thatthe population originated from Phong Dien and Quang Dien has the highest genetic Genetic … Dung Tran et al. identity, while the fish originated from Phu Vang and A Luoi showed the greatest genetic distance.

Thể loại: Quốc tế

Tác giả: Trần Quốc Dung, Mai Thế Hùng Anh, Hoàng Tấn Quảng, Trần Văn Giang, Vũ Thị Phương Anh

Đơn vị: Phòng thí nghiệm Công nghệ Gen

Đăng tại: Journal of Chemical, Biological and Physical Sciences An International Peer Review E-3 (2249 –1929)

Tập, (số), trang: 8, 1, 106-119

Số ISSN/ISBN: 2249-1929

Chỉ số Impact factor:

Điểm số theo QĐ của HĐ chức danh GS Nhà nước:

Năm công bố: 2017

File đính kèm: