Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochate chrysosporium.

Nội dung tóm tắt: The cDNA of acetyl xylan esterase 2 (PcAxe2) gene containing a carbohydrate binding module (CBM) sequence from Phanerochaete chrysosporium was cloned and expressed in Pichia pastoris. The recombinant PcAxe2 protein (rPcAxe2) was efficiently produced, reaching a maximum of 1058 U lL1 after 6 days of cultivation. Molecular mass of the rPcAxe2 on SDS-PAGE was approximately 63 kDa under hyperglycosylation. Optimal activity of the purified rPcAxe2 enzyme was observed at pH and temperature of 7.0 and 30e35 C, respectively. In addition to acetyl xylan esterase activity, rPcAxe2 also exhibited a xylanase activity at an optimum pH and temperature of 5.0 and 80 C, respectively. The synergistic action of rPcAxe2 with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan enhanced the total reducing soluble sugar.

Thể loại: Không thuộc ISI/SCOPUS, có ISSN/ISBN

Tác giả: Nguyễn Đức Huy, Saravanakumar Thiyagarajan, Dae-Hyuk Kim, Seung-Moon Park

Đơn vị: Ban lãnh đạo viện

Đăng tại: Journal of Bioscience and Bioengineering

Tập, (số), trang: 115, 5, 507-513

Số ISSN/ISBN: 1389 - 1723, E - ISSN: 1347-4421

Chỉ số Impact factor:

Điểm số theo QĐ của HĐ chức danh GS Nhà nước:

Năm công bố: 2013

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