Production of recombinant NPRC10 protease in 14-L fermentation scale

Nội dung tóm tắt: Present investigation describes the production of neutral protease (NPRC10) by recombinant E. coli BL21 (DE3) through submerged culture in 14-L fermenter. Different cultural parameters such as aeration rate, agitation speed and inoculum size were optimized for the production of enzyme. It was found that the maximum production of NPRC10 was obtained after 44 h of batch fermentation at aeration rate of 4 L/min, agitation speed of 500 rpm and inoculum size of 1%. The highest activity of protease during the time course of batch fermentation was 103 unit/mL.

Thể loại: Quốc tế

Tác giả: Nguyễn Hoàng Lộc, Nguyen Sy Cong, Hoàng Tấn Quảng, Nguyễn Đức Huy

Đơn vị: Phòng thí nghiệm Công nghệ Gen, Ban lãnh đạo viện

Đăng tại: European Journal of Experimental Biology

Tập, (số), trang: 2, 4, 913-918

Số ISSN/ISBN: 2248 –9215

Chỉ số Impact factor:

Điểm số theo QĐ của HĐ chức danh GS Nhà nước:

Năm công bố: 2012

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