In vivo Evaluation of Transgenic Watercress Containing Gene Encoding Escherichia coli Heat-Labile Toxin B Subunit

Nội dung tóm tắt: In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg g-1), photosynthetic rate (23.39 mgCO2 [dm2]-1 h-1), dry matter (7.42%), vitamin C (0.34 mg g-1), calcium (0.83 mg g-1), and potassium (2.47 mg g-1). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1-2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46% of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors.

Thể loại: Không thuộc ISI/SCOPUS, có ISSN/ISBN

Tác giả: Nguyễn Hoàng Lộc, Nguyễn Văn Song, Đặng Thanh Long, Tae-Geum Kim, Moon-Sik Yang

Đơn vị: Phòng thí nghiệm Vi sinh vật học và Công nghệ lên men

Đăng tại: Journal of Plant Biochemistry and Biotechnology

Tập, (số), trang: 24, 2, 129-134

Số ISSN/ISBN:

Chỉ số Impact factor:

Điểm số theo QĐ của HĐ chức danh GS Nhà nước:

Năm công bố: 2015

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