In vitro propagation of Adenosma indianum (Lour.) Merr.

Nội dung tóm tắt: This paper presents the results of in vitro propagation of Adenosma indianum (Lour.) Merr. The obtained results indicated that shoot regeneration from stem explant achieved on MS basal medium supplemented with 0.5 mg/L BAP after 4 weeks of culture, 46.7% of samples regenerating shoots with an average value of 1.8 shoots per sample. The MS medium containing 0.30 mg/L NAA was suitable for callus induction from leaves and containing 0.50 mg/L BAP and 0.10 mg/L NAA had a highest ability to produce buds from callus, with a ratio of 43.52%, and 3.43 shoots/callus. The multiplication coefficient was greatest on MS medium consist of 0.5 mg/L BAP and 0.3 mg/L IBA, average number of shoots per explant was 10.03 after 6 weeks of culture. The MS medium supplemented with 0.25 mg/L NAA was optimal for rooting (average value of 7.17 roots per shoot). The plantlets were acclimatized and transplanted successfully in the coconut coir pots, the plantlet survival rate was 91.67% after two months of cultivation. The in vitro propagation procedure of A. indianum (Lour.) Merr was developed successfully, shoots can be regenerated directly from axillary bud or callus and in vitro plantlets grow well in greenhouse.

Thể loại: Thuộc danh mục SCOPUS

Tác giả: Trương Thị Bích Phượng, Phan Thụy Trúc Như, Lâm Thị Ngọc Thủy, Trần Thúy Lan, Trần Quốc Dung, Hoàng Tấn Quảng

Đơn vị: Phòng thí nghiệm Công nghệ Gen

Đăng tại: Plant Cell Biotechnology and Molecular Biology

Tập, (số), trang: 20, 5&6, 222-231

Số ISSN/ISBN: 0972-2025

Chỉ số Impact factor:

Điểm số theo QĐ của HĐ chức danh GS Nhà nước:

Năm công bố: 2019

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