Bài báo khoa học: Micro-morphological, phytochemical and molecular characteristics of aquatic pennywort (Hydrocotyle verticillata) accessions in Vietnam
DOI: https://doi.org/10.21475/ajcs.25.19.01.p249
Tên tạp chí: Australian Journal of Crop Science
Author: Hai Thi Hong Truong, Nhi Thi Hoang Ho, Han Ngoc Ho, Thao Xuan Hoang
Abstract: Hydrocotyle verticillata, known as aquatic pennywort, is commonly found in wetlands. Recently, it is widely cultivated as an ornamental plant in Vietnam. In this work, we characterised a four-accession germplasm of H. verticillata, gathered across Vietnam with a maximal distance of 620 km. The accessions were identified based on ITS sequencing, using primers TSu1 and ITS2R. The four accessions displayed variations in morphological and micro-morphological traits such as stolon diameter, thickness of runner cortex, runner stele diameter, number of runner cortex cell layer, number of runner vascular bundles, diameter of runner xylem vessel elements, diameter of young root, root cortex thickness, diameter of root stele, number of cortex cell layer of root, number of xylem bundles of root, diameter of xylem vessel elements of root, leaf thickness and palisade mesophyll thickness. In addition, phytochemical contents, including Vitamin C, phenolic, flavonoid and saponin, were found to be significantly different among H. verticillata accessions, while reducing sugar, carotenoid and tannin contents were comparable. Finally, genetic diversity was demonstrated within the germplasm using RAPD genotyping. While the number of accessions was small (four accessions), this report showcased the diversity of aquatic pennywort accessions in Vietnam, warranting further studies into this vegetatively propagated species. These findings formed the basis for further research on breeding, cultivation and extraction of bioactive compounds from H. verticillata.
Keywords: Aquatic pennywort, Hydrocotyle verticillata, morphological, micro-morphological, RAPD
