LABORATORY OF ENZYME AND PROTEIN TECHNOLOGY

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I. Staffs

Nguyen Duc Huy, PhD
Head of laboratory
Phone: +84 2343813988
Email: ndhuy@hueuni.edu.vn

 

II. Research interests

– Isolation and identification high enzyme production microorganisms.

– Application on using enzyme from microorganism for treatment environment pollution, aquaculture, enhance food storage and processing, and produce bio-fertilizer.

– Production and application of recombinant microbial enzymes.

– Identification and qualification metabolism of production pathway of enzyme from microorganism

– Enzyme structure, modeling and characterization.

– Enzyme immobilization and bio-functional membrane.

– Optimization fermentation technology and processing for enzyme and protein production.

– Knock out technology for bacterial and study the gene function.

– Microbial safety analysis on food and vegetable and GAP guideline for agricultural application.

 

III. Projects

– Hue University (2015-2017): Identification of manganese peroxidase fungi isolated in Thua Thien Hue. PI: Nguyen Duc Huy

– International (2016-2017): Peroxidases production from wood decay fungi using rice straw medium. PI: Nguyen Duc Huy

– International (2017-2018): Detoxification of antibiotics commonly using in aquaculture by Fusarium oxysporum. PI: Nguyen Duc Huy

– Hue University (2018-2019): Nghiên cứu tạo dòng và phân tích trình tự gen laccase từ Fusarium oxysporum HUIB02. PI: Nguyen Duc Huy

– International (2018-2019): Production of recombinant laccase and application on synthesis dyes degradation. PI: Nguyen Duc Huy

– Ministry of Education and Training (2018-2020): Development microorganism solution against Vibrio sp. for prevention and treatment diseases on shrimp, fishes. PI: Nguyen Duc Huy

IV. Publications

International journal

  1. Le Cong Tuan, Nguyen Duc Huy, Le My Tieu Ngoc, Doan Thi My Lanh, Te Minh Son, Nguyen Hoang Loc. 2021. Nitrogen removal efficiency of some bacterial strains isolated from seawater in Thua Thien Hue, Vietnam. Advancements in Life Sciences 8 (2), 184-189
  2. Nguyen Duc Huy, Nguyen Thi My Le, Kit Wayne Chew, Seung-Moon Park, Pau Loke Show. 2021. Characterization of a recombinant laccase from Fusarium oxysporum HUIB02 for biochemical application on dyes removal. Biochemical Engineering Journal, 107958
  3. Do Thi Bich Thuy, An Tien Nguyen, Kuan Shiong Khoo, Kit Wayne Chew, Margo Cnockaert, Peter Vandamme, Yeek-Chia Ho, Nguyen Duc Huy, Heriberto Hernández Cocoletzi, Pau Loke Show. 2021. Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from ‘mam nem’, a fermented fish sauce. Bioengineered 12 (1), 54-62
  4. Hoang Tan Quang, Pham Thi Diem Thi, Tran Thuy Lan, Nguyen Duc Huy, Nguyen Duy Quynh Tram, Nguyen Thi Thu Lien. 2020. Development of a diagnostic scar marker for Vibrio shilonii caused acute hepatopancreatic necrosis disease in whiteleg shrimp. Advancements in Life Sciences 7 (4), 257-263
  5. Nguyen Duc Huy, Dang Thi Thanh Ha, Kuan Shiong Khoo, Pham Thi Ngoc Lan, Hoang Tan Quang, Nguyen Hoang Loc, Seung-Moon Park, Ashokkumar Veeramuthu, Pau Loke Show. 2020. Synthetic dyes removal by Fusarium oxysporum HUIB02 and stimulation effect on laccase accumulation. Environmental Technology & Innovation 19, 101027
  6. Chung D. Ngo, Phuong L. T. Le, Huy D. Nguyen, Phong B. Truong, Nghiep T. Hoang, and Binh V. Ngo. 2020. Diet of the Bronze Skink Eutropis macularius (Reptilia: Squamata: Scincidae) from Thua Thien Hue Province, Central Vietnam. Russian Journal of Herpetology 27 (4)
  7. Nguyen Duc Huy, Thiyagarajan Saravanakumar, Sang Hoon Ha & Seung-Moon Park. 2020. Enhanced enzymatic saccharification of wheat flour arabinoxylan and barley straw using recombinant hemicellulases. Biotechnology and Bioprocess Engineering 25 (3), 431-441
  8. Nguyen Duc Huy, Le My Tieu Ngoc, Nguyen Hoang Loc, Tran Thuy Lan, Hoang Tan Quang, Tran Quoc Dung, Truong Thi Phuong Lan, Vu Duc Hoang and Nguyen Thi Dong Phuong. 2020. Isolation of Weissella cibaria from Pacific White Shrimp (Litopenaeus vannamei) Gastrointestinal Tract and Evaluation of Its Pathogenic Bacterial Inhibition. Indian Journal of Science and Technology 13 (10), 1200 – 1212
  9. Nguyen Hoang Loc, Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan and Tran Thi Thu Ha. 2020. Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum PQ34. Mycology 11 (1), 38-48
  10. Thu, Nguyen T.A.; Khue, Nguyen T.M.; Huy, Nguyen D.; Tien, Nguyen Q.D.; Loc, Nguyen H. 2020. Characterizations and Fibrinolytic Activity of Serine Protease from Bacillus subtilis C10. Current Pharmaceutical Biotechnology 21 (2), 110-116(7)
  11. Truong Thi Phuong Lan, Nguyen Duc Huy, Nguyen Ngoc Luong, Hoang Tan Quang, Trinh Huu Tan, Le Thi Anh Thu, Nguyen Xuan Huy, Nguyen Hoang Loc. 2019. Effect of salicylic acid and yeast extract on curcuminoids biosynthesis gene expression and curcumin accumulation in cells of Curcuma zedoaria. Journal of Plant Biotechnology 46 (3), 172-179
  12. Thi Dong Phuong Nguyen, Duc Huy Nguyen, Jun Wei Lim, Chih-Kai Chang, Hui Yi Leong, Thi Ngoc Thu Tran, Thi Bich Hau Vu, Thi Trung Chinh Nguyen and Pau Loke Show. 2019. Investigation of the Relationship between Bacteria Growth and Lipid Production Cultivating of Microalgae Chlorella Vulgaris in Seafood Wastewater. Energies 12, 2282
  13. Tran Vu Ngoc Thi, Duong Duc Hoang Sinh, Le Thi Ha Thanh, Nguyen Duc Huy, Nguyen Hoang Tue, Masaki Shintani, Kazuhide Kimbara, Nguyen Hoang Loc. 2018. Cloning, expression and characterization of catechol 1, 2-dioxygenase from Burkholderia cepacia. The Journal of general and applied microbiology. DOI: https://doi.org/10.2323/jgam.2019.06.002
  14. Lan, Truong T. P.; Huy, Nguyen D.; Luong, Nguyen Ngoc; Nghi, Nguyen Van; Tan, Trinh Huu; Quan, Le Viet; Loc, Nguyen Hoang. 2018. Identification and Characterization of Genes in the Curcuminoid Pathway of Curcuma zedoaria Roscoe. Current pharmaceutical biotechnology 19 (10), 839-846
  15. Nguyen Thi Anh Thu, Ngo Thi Tuong Chau, Le Van Thien, Nguyen Duc Huy, Nguyen Tran Me Khue, Nguyen Bao Hung, Nguyen Ngoc Luong, Le Thi Anh Thu, Nguyen Hoang Loc. 2018. Extrachromosomal expression of nat05 gene encoding an alkaline serine protease from Bacillus subtilis N05. Journal of Biotechnology, Computational Biology and Bionanotechnology. DOI: https://doi.org/10.5114/bta.2018.79967
  16. Nguyen Duc Huy, Nguyen Thi Thanh Tien, Le Thi Huyen, Hoang Tan Quang, Truong Quy Tung, Nguyen Ngoc Luong, Seung-Moon Park. 2017. Screening and Production of Manganese Peroxidase from Fusarium sp. on Residue Materials. Mycobiology. 45(1): 52-56.
  17. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Phuong Lan. 2017. Accumulation of madecassoside – a major component of centelloside – in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid. Periodicum Biologorum 119(1): 81-85.
  18. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy. 2016. Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (Centella asiatica (L.) Urban) cells. 3 Biotech. 6(1): 86
  19. Nguyen Hoang Loc, Le My Tieu Ngoc, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Ngoc Luong. 2016. Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris. Chemical Papers 70(3): 284–293.
  20. Nguyen DucHuy, Cu Le Nguyen, Han-Sung Park, Nguyen Hoang Loc, Myoung-Suk Choi, Dae-Hyuk Kim, Jeong-Woo Seo, Seung-Moon Park. 2016. Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium. Journal of Bioscience and Bioengineering 121(2): 154-159
  21. Nguyen Thanh Giang, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Huu Thuan Anh, Truong Thi Phuong Lan, Nguyen Hoang Loc. 2016. Effect of elicitor on asiaticoside accumulation in Centella (Centella asiatica (L.) urban) cells. Vietnam National University Journal of Science 21 (4S), 44-49
  22. Hoang Tan Quang, Cai Thi Quynh Thoa, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Bich Phuong and Van Thi Yen. 2016. Study on genetic diversity of Paris polyphylla population from Vietnam and China. Plant Cell Biotechnology and Molecular Biology 17 (1-2), 57-63
  23. Nguyen Thanh Giang, Nguyen Duc Huy, Le The Ha Thanh, Nguyen Hoang Loc. 2016. Efficiency of the combination of salicylic acid, yeast extract and methyl jasmonate on asiaticoside production in centella (Centella asiatica (L.) urban) cells. Plant Cell Biotechnology and Molecular Biology. 17(1-2): 64-71.

Domestic journal

  1. Le Cong Tuan, Te Minh Son, Doan Thi My Lanh, Nguyen Hoang Loc, Nguyen Duc Huy. 2021. Evaluation of biofloc from saltwater bacteria community for white-leg shrimp (Litopenaeus vanamei Boone, 1931) culture in Thua Thien Hue. Hue University Journal of Science: Natural Science 130 (1A): 117-123
  2. Lê Mỹ Tiểu Ngọc, Đặng Quang Nguyên, Đỗ Trần Hương Duyên, Trần Thúy Lan, Nguyễn Quang Đức Tiến, Nguyễn Duy Quỳnh Trâm, Nguyễn Đức Huy. 2019. Isolation and evaluation anti-pathogenicactivities of Lactococcus garvieae from shrimp intestinalsystem. Hue University Journal of Science: Natural Science 128 (1E), 77-86
  3. Duong Duc Hoang Sinh, Tran Vu Ngoc Thi, Le Thi Ha Thanh, Phạm Thị Ngọc Lan, Nguyen Hoang Loc, Nguyen Duc Huy. 2018. Isolation of some bacterial strains from dioxin-contaminated soil in A Luoi, Thua Thien Hue. Hue University Journal of Science: Natural Science 127 (1C), 141–148
  4. Dang Thi Thanh Ha, Le Kim Tuan, Dinh Thi Kim Thien, Pham Thi Ngoc Lan, Hoang Tan Quang, Tran Thuy Lan, Nguyen Duc Huy. 2018. Cloning and sequencinglaccase 3 (Folac3) from Fusarium oxysporum. Hue University Journal of Science: Natural Science 127 (1C), 1-10
  5. Hoang Tan Quang, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Phuong Lan. 2016. Effects of methyl jasmonate on saponin accumulation in cultured suspension cells of Paris polyphylla Smith. Proceeding of the 2nd national conference on biological research and teaching in Vietnam, 1176-1183

 Scientific awards

1. Third award from ThuaThienHue contest on Science and Technology, 2018

Title: Identification manganese peroxidase production fungi from Thua Thien Hue

Author: Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Le Thi Dieu Huyen, Nguyen Thi Thanh Tuyen, Nguyen Thi Be

2. 2017 Co Do award in science and technology

Title: Development and application of recombinant microbial enzymes.

Author: Nguyen Hoang Loc, Do Thi Bich Thuy, Tran Thi Thu Ha, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Bao Hung, Nguyen Thi Quy Hoa, Le My Tieu Ngoc, Nguyen Ngoc Luong

Technology transfer products

 

 

English