On July 3rd 2020, Institute of Biotechnology, Hue University (HUIB) organized the Scientific Committee to evaluate the Hue University research project “Cloning and sequence analysis of laccase coding gene from Fusarium oxysporum HUIB02”, code: DHH2018-15-09 was headed by Dr. Nguyen Duc Huy, HUIB was the implementation institution.
At the meeting, on behalf of the research team, Dr. Nguyen Duc Huy presented of the process, and key results of the project, such as: amplification Folac1, Folac2, and Folac3 genes from cDNA and DNA genome of Fusarium oxysporum HUIB02, these genes have been deposited on Genbank database: Folac1 (MT362052), Folac2 (KY825237), and Folac3 (KY825238); cloning Folac1, Folac2, and Folac3 genes into pGEM®T-Easy vector; Folac1 sequence consists of 2064 nucleotides, encoding for an amino acids chain of 686 aa, protein structure analysis demonstrates Folac1 is extracellular enzyme with a signal peptide and 11 N-glycosylation positions, secondary structure contains 7 α-chains and 38 β-chains; Folac2 sequence consists of 2229 nucleotides, encoding for an amino acids chain of 645 aa, protein structure analysis demonstrates Folac2 is extracellular enzyme with a signal peptide and 12 N-glycosylation positions, secondary structure contains 7 α-chains and 36 β-chains; Folac3 sequence consists of 1957 nucleotides, encoding for an amino acids chain of 617 aa, protein structure analysis demonstrates Folac3 is intracellular enzyme with 7 N-glycosylation positions, secondary structure contains 6 α-chains and 30 β-chains. The research team published 01 article in Hue University Journal of Science, 03 nucleotide sequences on Genbank database, and supported to train 02 engineers in Biotechnology.
Members of the scientific evaluation council highly appreciated the results of project and commented on some modification (form errors, scientific terms…) and correction on the contents of final report.
Assoc. Prof. Tran Quoc Dung – Chairmain of the Scientific Committee agreed with the comments and assessments of the reviewers and members of the Scientific Committee, and suggested that the research team should correct the report.
The evaluation of research project: Good
Dr. Nguyen Duc Huy presenting at the meeting