LABORATORY OF ENZYME AND PROTEIN TECHNOLOGY

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I. NAME

Laboratory of Enzyme and Protein Technology

Dr. Nguyen Duc Huy

Head of laboratory

Lecturer

Phone number: (+84) 0916 066 357
Email: ndhuy@hueuni.edu.vn

II. STAFF

No. Name Degree Major Job position Phone number Email
1 Nguyen Duc Huy Doctor Biotechnology Head of Lab. 0916066357 ndhuy@hueuni.edu.vn
2 Nguyen Thi Minh Nga Engineer Biotechnology Researcher 0775581698 nguyenthiminhnga@hueuni.edu.vn

III. RESEARCH INTERESTS

– Isolation and identification high enzyme production microorganisms.

– Application on using enzyme from microorganism for treatment environment pollution, aquaculture, enhance food storage and processing, and produce bio-fertilizer.

– Production and application of recombinant microbial enzymes.

– Identification and qualification metabolism of production pathway of enzyme from microorganism.

– Enzyme structure, modeling and characterization.

– Enzyme immobilization and bio-functional membrane.

– Optimization fermentation technology and processing for enzyme and protein production.

– Knock out technology for bacterial and study the gene function.

– Microbial safety analysis on food and vegetable and GAP guideline for agricultural application.


IV. PROJECTS

– Hue University (2015 – 2017): Identification of manganese peroxidase fungi isolated in Thua Thien Hue. PI: Nguyen Duc Huy.

– International (2016 – 2017): Peroxidases production from wood decay fungi using rice straw medium. PI: Nguyen Duc Huy.

– International (2017 – 2018): Detoxification of antibiotics commonly using in aquaculture by Fusarium oxysporum. PI: Nguyen Duc Huy.

– Hue University (2018 – 2019): Cloning and sequence analysis of laccase gene from Fusarium oxysporum HUIB02. PI: Nguyen Duc Huy.

– International (2018 – 2019): Production of recombinant laccase and application on synthesis dyes degradation. Chủ nhiệm: PI: Nguyen Duc Huy.

– Ministry of Education and Training (2018 – 2020): Development microorganism solution against Vibrio sp. for prevention and treatment diseases on shrimp, fishes. PI: Nguyen Duc Huy.


V. SCIENTIFIC PUBLICATION 

  1. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy , Truong Thi Phuong Lan. 2017. Accumulation of madecassoside – a major component of centelloside – in centella ( Centella asiatica (L.) Urban) cells elicited by salicylic acid. Periodicum Biologorum. 119(1): 81-85.
  2. Nguyen Duc Huy , Nguyen Thi Thanh Tien, Le Thi Huyen, Hoang Tan Quang, Truong Quy Tung, Nguyen Ngoc Luong, Seung-Moon Park. 2017. Screening and production of manganese peroxidase from Fusarium on residue materials. Mycobiology . 45(1): 52-56.
  3. Dang Thi Thanh Ha, Le Kim Tuan, Dinh Thi Kim Thien, Pham Thi Ngoc Lan, Hoang Tan Quang, Tran Thuy Lan, Nguyen Duc Huy. 2018. Cloning and sequencing laccase 3 ( Folac3 ) from Fusarium oxysporum. Hue University Journal of Science. 127(1C): 1-10.
  4. Duong Duc Hoang Sinh, Tran Vu Ngoc Thi, Le Thi Ha Thanh, Pham Thi Ngoc Lan, Nguyen Hoang Loc, Nguyen Duc Huy. 2018. Isolation of some bacterial strains from dioxin-contaminated soil in A luoi, Thua Thien Hue. Hue University Journal of Science. 127(1C): 141–148.
  5. Truong Thi Phuong Lan, Nguyen Duc Huy , Nguyen Ngoc Luong, Nguyen Van Nghi, Trinh Huu Tan, Le Viet Quan, Nguyen Hoang Loc 2018. Identification and characterization of genes in curcuminoid pathway of Curcuma zedoaria Current Pharmaceutical Biotechnology. 19(10):839-846.
  6. Nguyen Thi Anh Thu, Ngo Thi Tuong Chau, Le Van Thien, Nguyen Duc Huy , Nguyen Tran Me Khue, Nguyen Bao Hung, Nguyen Ngoc Luong, Le Thi Anh Thu, Nguyen Hoang Loc. 2018. Extra-chromosomal expression of nat05 gene encoding an alkaline serine protease from Bacillus subtilis Biotechnologia. 99(4): 365–373.
  7. Thi Dong Phuong Nguyen, Duc Huy Nguyen , Jun Wei Lim, Chih-Kai Chang, Hui Yi Leong, Thi Ngoc Thu Tran, Thi Bich Hau Vu, Thi Trung Chinh Nguyen and Pau Loke Show. 2019. Investigation of the relationship between bacteria growth and lipid production of microalgae chlorella vulgaris in seafood wastewater. Energies. 12(12): 2282.
  8. Le My Tieu Ngoc, Dang Quang Nguyen, Do Tran Huong Duyen, Tran Thuy Lan, Nguyen Quang Duc Tien, Nguyen Duy Quynh Tram, Nguyen Duc Huy . 2019. Isolation and evaluation anti-pathogenic activities of Lactococcus garvieae from shrimp intestinal system. Hue University Journal of Science. 128(1E): 77-86.
  9. Nguyen Hoang Loc, Nguyen Duc Huy , Hoang Tan Quang, Tran Thuy Lan, Tran Thi Thu Ha. 2019. Characterization and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum Mycology. 11(1): 38-48.
  10. Tran Vu Ngoc Thi, Duong Duc Hoang Sinh, Le Thi Ha Thanh, Nguyen Duc Huy , Nguyen Hoang Tue, Masaki Shintani, Kazuhide Kimbara , Nguyen Hoang Loc. 2020. Cloning, expression and characterization of catechol 1,2-dioxygenase from Burkholderia cepacia. Journal of General and Applied Microbiology. 66(3):188-194.
  11. Hoang Tan Quang, Pham Thi Diem Thi, Tran Thuy Lan, Nguyen Duc Huy , Nguyen Duy Quynh Tram, Nguyen Thi Thu Lien. 2020. Development of a diagnostic scar marker for Vibrio shilonii caused acute hepatopancreatic necrosis disease in whiteleg shrimp. Advancements in Life Sciences. 7(4): 257-263.
  12. Nguyen Duc Huy , Dang Thi Thanh Ha, Kuan Shiong Khoo, Pham Thi Ngoc Lan, Hoang Tan Quang, Nguyen Hoang Loc, Seung-Moon Park, Ashokkumar Veeramuthu, Pau Loke Show. 2020. Synthetic dyes removal by Fusarium oxysporum HUIB02 and stimulation effect on laccase accumulation. Environmental Technology & Innovation. 101027.
  13. Nguyen Duc Huy , Thiyagarajan Saravanakumar, Sang Hoon Ha, Seung-Moon Park. 2020. Enhanced enzymatic saccharification of wheat flour arabinoxylan and barley straw using recombinant hemicellulases. Biotechnology and Bioprocess Engineering. 25(3): 431-441.
  14. Chung D Ngo, Phuong LT Le, Huy D Nguyen , Phong B Truong, Nghiep T Hoang, Binh V Ngo. 2020. Diet of the bronze skink Eutropis macularius (reptilia: squamata: scincidae) from Thua Thien Hue province, Central Vietnam. 27(4): 209-216.
  15. Nguyen Hoang Loc, Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Tran Thi Thu Ha. 2020. Characterization and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum 11(1): 38-48.
  16. Nguyen Duc Huy , Le My Tieu Ngoc, Nguyen Hoang Loc, Tran Thuy Lan, Hoang Tan Quang, Tran Quoc Dung, Truong Thi Phuong Lan, Vu Duc Hoang and Nguyen Thi Dong Phuong. 2020. Isolation of Weissella cibaria from Pacific white shrimp ( Litopenaeus vannamei ) gastrointestinal tract and evaluation of its pathogenic bacterial inhibition. Indian Journal of Science and Technology. 13(10): 1200 – 1212.
  17. Nguyen Thi Anh Thu, Nguyen Tran Me Khue, Nguyen Duc Huy , Nguyen Quang Duc Tien, Nguyen Hoang Loc. 2020. Characterizations and fibrinolytic activity of serine protease from Bacillus subtilis Current Pharmaceutical Biotechnology. 21(2): 110-116.
  18. Le Cong Tuan, Nguyen Duc Huy , Le My Tieu Ngoc, Doan Thi My Lanh, Te Minh Son, Nguyen Hoang Loc. 2021. Nitrogen removal efficiency of some bacterial strains isolated from seawater in Thua Thien Hue, Vietnam. 8(2):184-189.
  19. Nguyen Duc Huy , Nguyen Thi My Le, Kit Wayne Chew, Seung-Moon Park, Pau Loke Show. 2021. Characterization of a recombinant laccase from Fusarium oxysporum HUIB02 for biochemical application on dyes removal. Biochemical Engineering Journal. 107958.
  20. Do Thi Bich Thuy, AnTien Nguyen, Kuan Shiong Khoo, Kit Wayne Chew, Margo Cnockaert, Peter Vandamme, Yeek-Chia Ho, Nguyen Duc Huy , Heriberto Hernández Cocoletzi, Pau Loke Show. 2021. Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from ‘mam nem’, a fermented fish sauce. Bioengineered. 12(1):54-62.
  21. Hooi Ren Lim, Kuan Shiong Khoo, Kit Wayne Chew, Chih-Kai Chang, Heli Siti Halimatul Munawaroh, P Senthil Kumar, Nguyen Duc Huy , Pau Loke Show. 2021. Perspective of Spirulina culture with wastewater into a sustainable circular bioeconomy. Environmental Pollution. 284: 117492.
  22. Trinh Thi Phuong Thao, Le Thi Kim Thoa, Le My Tieu Ngoc, Truong Thi Phuong Lan, Tran Vinh Phuong, Hai Thi Hong Truong, Kuan Shiong Khoo, Sivakumar Manickam, Truong Thi Hoa, Nguyen Duy Quynh Tram, Pau Loke Show, Nguyen Duc Huy . 2021. Characterization halotolerant lactic acid bacteria Pediococcus pentosaceus HN10 and in vivo evaluation for bacterial pathogens inhibition. Chemical Engineering and Processing-Process Intensification. 168: 108576.
  23. Le Thi Kim Thoa, Trinh Thi Phuong Thao, Nguyen Bao Hung, Kuan Shiong Khoo, Hoang Tan Quang, Tran Thuy Lan, Vu Duc Hoang, Seung-Moon Park, Chien Wei Ooi, Pau Loke Show, Nguyen Duc Huy. 2022. Biodegradation and detoxification of malachite green by extracellular laccase expressed from Fusarium oxysporum. Waste and Biomass Valorization . https://doi.org/10.1007/s12649-022-01692-2.
  24. Nguyen Duc Huy, Thao Trinh Thi Phuong, Thoa Le Thi Kim, Vu Nguyen Quang Hoang, Lich Nguyen Quang, Tuan Le Cong, Binh Tran Dinh, Anh Nguyen Thi Chau, Lan Truong Thi Phuong, Park Seung-Moon. 2022. Assessment of antioxidant and antibacterial activities of Lactobacillus farciminis HN11 as a probiotic candidate. Applied Food Biotechnology , 9(2), 103-112.

VI. SCIENCE AND TECHNOLOGY AWARD 

6.1. 2017 Co Do award in science and technology

Title: Development and application of recombinant microbial enzymes.

Author: Nguyen Hoang Loc, Do Thi Bich Thuy, Tran Thi Thu Ha, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Bao Hung, Nguyen Thi Quy Hoa, Le My Tieu Ngoc, Nguyen Ngoc Luong

6.2. Third award from ThuaThienHue contest on Science and Technology, 2018  

Title: Identification manganese peroxidase production fungi from Thua Thien Hue

Author: Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Le Thi Dieu Huyen, Nguyen Thi Thanh Tuyen, Nguyen Thi Be

6.3. Consolation prize from Thua Thien Hue contest on Science and Technology, 2020

Title: Research on creating antagonistic microbial products Vibrio sp. for the prevention and treatment of shrimp diseases

Author: Nguyen Duc Huy, Le My Tieu Ngoc, Do Tran Huong Duyen, Hoang Tan Quang, Nguyen Bao Hung, Tran Thuy Lan, Nguyen Van Khanh, Le Thi Kim Thoa, Trinh Thi Phuong Thao

English