Production of recombinant NPRC10 protease in 14-L fermentation scale
Summary: Present investigation describes the production of neutral protease (NPRC10) by recombinant E. coli BL21 (DE3) through submerged culture in 14-L fermenter. Different cultural parameters such as aeration rate, agitation speed and inoculum size were optimized for the production of enzyme. It was found that the maximum production of NPRC10 was obtained after 44 h of batch fermentation at aeration rate of 4 L/min, agitation speed of 500 rpm and inoculum size of 1%. The highest activity of protease during the time course of batch fermentation was 103 unit/mL.
Type: Non-ISI/SCOPUS listed journals with ISSB/ISBN
Author: Nguyễn Hoàng Lộc, Nguyen Sy Cong, Hoàng Tấn Quảng, Nguyễn Đức Huy
Unit: Laboratory of Gene technology, Director board of Institute
Journal:European Journal of Experimental Biology
Issue, Number, Pages2, 4, 913-918
ISSN/ISBN:2248 –9215
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Year of publication: 2012
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