Expression of chitinase (chi42) gene from Trichoderma asperellum in Saccharomyces cerevisiae
Summary: We cloned a gene coding chitinase (chi42) from strains Trichoderma asperellum SH 16 isolated from Thua Thien Hue province, Vietnam. Expression of the chitinase in Saccharomyces cerevisiae INVSc1 produced a fusion protein of approximately 50 kDa (42 kDa of mature chitinase, 4 kDa of signal peptide and 3.4 kDa of fusion tag of pYES2/NT(C)/chi42 vector). The effect of induction time on the chitinase production was investigated. The results showed that the highest total activity (0.012 unit/mL) of chitinase occurred after 12 h of induction with 2% galactose
Type: Non-ISI/SCOPUS listed journals with ISSB/ISBN
Author: Nguyễn Hoàng Lộc, Pham Thi Kim Cuc, Hoàng Tấn Quảng
Unit: Laboratory of Gene technology
Journal:Annals of Biological Research
Issue, Number, Pages4, 9, 15-19
ISSN/ISBN:0976 - 1233
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Year of publication: 2013
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