Construction of a Genetic Linkage Map for MappingPhytophthora capsici Resistance Using ‘YCM334 x Tean’Recombinant Inbred Line Population
Summary: Phytophthora capcisi Leonian, known as the causal agent of the stem, collar and root rot, is one of the most serious problem limiting the pepper production in many areas in the world. Genetic resistance to the parasite displays complex inheritance. To date, no pepper accession showing complete resistance to Phytophtora wilt have been found. A number of C. annuum accessions were reported to be partially resistant to P. capsici. The accession Criollo de Morelos 334 (CM334), considered as being the most efficient source of resistance currently known, is largely used in breeding programs. Several conflicting genetic controls have been suggested, going from two recessive genes to QTLs with epistatic effects. To elucidate genetic control of resistance in CM334, we constructed a genetic linkage map for further QTL analysis using a population of 126 F6 recombinant inbred lines (RILs) derived from a cross between C. annuum YCM334 (resistance parent) and C. annuum Tean (susceptible parent). A total of 438 markers included 286 AFLPs, 25 SSRs, 111 unmapped SSR, 1 CAPS and 15 SRAPs were used for map construction. Of those, 187 AFLPs, 20 SSRs, 78 unmapped SSRs, and 8 SRAPs were mapped and split into 17 linakge groups. Thirteen linkage groups were assigned into 11 chromosomes (1, 2, 3, 4, 5, 6, 7, 8, 10, 11, and 12). The map covered 1941.4 cM with an average of 6.6 cM. The map will be used for further QTL analysis stud
Type: Non-ISI/SCOPUS listed journals with ISSB/ISBN
Author: Trương Thị Hồng Hải, JH. Park, Y. Chea
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Journal:Korean Journal Horticulture Science Technology
Issue, Number, Pages27, Supply II, 83
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Year of publication: 2009
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