LABORATORY OF GENE TECHNOLOGY

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I. NAME

Laboratory of Gene Technology

Dr. Hoang Tan Quang

Head of Laboratory

Phone number: (+84) 0983 735 509

Email: htquang@hueuni.edu.vn

II. HISTORY

• In 2003, Hue University set up a Laboratory of Molecular Biotechnology, belong to the Center for Resources, Environment and Biotechnology (CREB), Hue University (located at 27 Phan Dinh Phung Str., Hue city).
• In November 2007, the Institute of Resources, Environment and Biotechnology (IREB) was established based on the reorganization of CREB, Laboratory of Molecular Biotechnology became the Department of Applied DNA Technology.
• In 2011, the department was renamed to the Department of Molecular Biology.
• In April 2013, the Department was separated from IREB and belonged to Center for Scientific Incubation and Transfer (CSIT), Hue University.
• In May 2014, the Institute of Biotechnology (HUIB) was re-established and the Department of Molecular Biology was re-established.
• In April 2019, the department was renamed to Laboratory of Gene Technology.

III. STAFFS

IV. RESEARCH INTERESTS

• Production of biological products using recombinant DNA technology for agriculture.
• Biodiversity of valuable genetic resources.
• Gene expression regulation in plant and microorganism.
• Conservation and propagation of valuable medicine plants.
• Production of bioactive compounds based on plant cells tissue culture.

V. PROJECTS

– Hue University (2018-2019): Research on in vitro propagation of Adenosma indianum (Lour.) Merr. PI. Hoang Tan Quang.

– Ministry of Education and Traning (2019-2020): Production of gypenoside from cell suspension culture of Gynostemma pentaphyllum (Thunb.) Makino. PI. Hoang Tan Quang.

– Hue University (2021-2022): Study on bio-antagonism potential of fungi strains resistant to Phytophthora sp. on pomelo. PI. Pham Thi Diem Thi.

– Ministry of Education and Traning (2022-2023): Research and development of microbial preparations with high β-1,3-glucanase activity against Colletotrichum spp. on chili. PI. Pham Thi Diem Thi.

VI. PUBLICATION 

1. Van-Thuan Nguyen, Van-Giang Tran, Tran-Trung Nguyen, Tan-Quang Hoang, Quoc-Dung Tran (2018) Genetic diversity of earthworm Amynthas rodericensis (Grube, 1879) (Clitellata: Megascolecidae) in Vietnam by randomly amplified polymorphic DNA analysis. Journal of Chemical, Biological and Physical Sciences. Section B 8(4): 870-883.
2. Hoang Tan Quang, Bui Le Thanh Nhan, Tran Minh Duc, Tran Van Giang, Pham Thanh (2018) Study on genetic diversity of Curculigo orchioides Gaertn populations from Vietnam, an endangered medicinal herb. Plant Cell Biotechnology and Molecular Biology 19(7-8): 293-301.
3. Dung Q. Tran, Thien V. Tran, Quang T. Hoang (2018) Genetic Relationship of Two Agamid Lizard Species in Vietnam by Random Amplified Polymorphic DNA Analysis. Life Science Journal 15(10): 36-42.
4. Nguyen Quang Duc Tien, Le Quang Man, Ngo Thi Ly, Duong Thi Kim Chi, Tran Thuy Lan, Nguyen Xuan Huy (2019) Cloning, expression, and purification of truncated S1 epitope and peptide CT24 fusion protein of porcine epidemic diarrhea virus in Escherichia coli. Plant Cell Biotechnology and Molecular Biology 20(3-4): 112-118.
5. Tae-Geum Kim, Tran Thuy Lan, Jin-Yong Lee (2019) Immunogenicity of Fusion Protein of Cholera Toxin B Subunit-Porphyromonas gingivalis 53-kDa Minor Fimbrial Protein Produced in Nicotiana benthamiana. Biotechnol Bioproc E 24, 954–963.
6. Truong Thi Bich Phuong, Phan Thuy Truc Nhu, Lam Thi Ngoc Thuy, Tran Thuy Lan, Tran Quoc Dung, Hoang Tan Quang (2019) In vitro propagation of Adenosma indianum (Lour.) Merr. Plant Cell Biotechnology and Molecular Biology 20(5-6): 222-231.
7. Truong Thi Phuong Lan, Nguyen Duc Huy, Nguyen Ngoc Luong, Hoang Tan Quang, Trinh Huu Tan, Le Thi Anh Thu, Nguyen Xuan Huy, Nguyen Hoang Loc (2019) Expression of elicitor-induced curcuminoid genes in cells of zedoary (Curcuma zedoariaRoscoe). Journal of Plant Biotechnology 46:172-179.
8. Nguyen Hoang An, Tran Thi Minh Chien, Ho Thi Hoang Nhi, Nguyen Thi Minh Nga, Tran Thien Phuc, Lam Thi Ngoc Thuy, Tong Van Bao Thanh, Phan Thi Thao Nguyen, Truong Thi Bich Phuong (2020). The effects of sucrose, silver nitrate, plant growth regulators, and ammonium nitrate on microrhizome induction in perennially-cultivated ginger (Zingiber officinale Roscoe) from Hue, Vietnam. Acta Agrobot. Artic, 73, 1-11.
9. Nguyen Hoang Loc, Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan,Tran Thi Thu Ha (2020) Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus. Trichoderma asperellum PQ34. Mycology 11(1): 38-48, DOI: 10.1080/21501203.2019.1703839
10. Nguyen Duc Huy, Le My Tieu Ngoc, Nguyen Hoang Loc, Tran Thuy Lan, Hoang Tan Quang, Tran Quoc Dung, Truong Thi Phuong Lan, Vu Duc Hoang, Nguyen Thi Dong Phuong (2020) Isolation of Weissella cibariafrom Pacific white shrimp (Litopenaeus vannamei) gastrointestinal tract and evaluation of its pathogenic bacterial inhibition. Indian Journal of Science and Technology: 13(10), 1200-1212. DOI: 10.17485/ijst/2020/v013i10/149934.
11. Hoang Tan Quang, Tran Thuy Lan, Truong Thi Hong Hai, Pham Thi Hai Yen, Tran Quang Khanh Van, Ho Thi Tung, Mac Nhu Binh, Nguyen Khoa Huy Son, Nguyen Quang Linh, Nguyen Duy Quynh Tram (2020) Genetic diversity and toxic genes analysis of Vibrio isolated from white leg shrimp and marine fishes cultured in Tam Giang lagoon in Thua Thien Hue province, Vietnam. Indian Journal of Science and Technology 13(13): 1412-1422. https://doi.org/10.17485/IJST/v13i13.161
12. Hoang Tan Quang, Pham Thi Diem Thi, Tran Thuy Lan, Nguyen Duc Huy, Nguyen Duy Quynh Tram, Nguyen Thi Thu Lien (2020) Development of a diagnostic SCAR marker for Vibrio shilonii caused acute hepatopancreatic necrosis disease in whiteleg shrimp. Advancements in Life Sciences 7(4): 257-263.
13. Nguyen Hoang Loc, Nguyen Hong Van, Hoang Tan Quang (2020) Characterization of carboxymethyl cellulase produced by Trichoderma asperellum. Advancements in Life Sciences 8(1): 42-46.
14. Nguyen Duc Huy, Dang Thi Thanh Ha, Kuan Shiong Khoo, Pham Thi Ngoc Lan, Hoang Tan Quang, Nguyen Hoang Loc, Seung-Moon Park, Ashokkumar Veeramuthu, Pau Loke Show (2020) Synthetic dyes removal by Fusarium oxysporum HUIB02 and stimulation effect on laccase accumulation. Environmental Technology & Innovation 19 (2020) 101027
15. Nguyen Hoang An, Phan Thi Thao Nguyen, Tran Thuy Lan, Hoang Tan Quang, Truong Thi Bich Phuong (2021) Genetic diversity analysis based on Retrotransposon Microsatellite Amplification Polymorphisms (REMAP) for distinguishing the ginger chemotype of Thua Thien Hue (Zingiber officinale Roscoe) from other Vietnamese ginger types. Jordan Journal of Biological Sciences 14(2).
16. Nguyen Quang Hoang Vu, Hoang Thi Kim Hong, Hoang Tan Quang (2021) Effects of different treatments of silver nanoparticles (AgNPs) on the growth & physiological characteristics of lotus (Nelumbo nucifera). IOP Conference Series: Earth and Environmental Science, 947(1), pp. 012038.
17. Nguyen Van Hue, Hoang Tan Quang, Dang Thanh Long, Nguyen Van Toan, Nguyen Thi Van Anh, Tran Thanh Quynh Anh, Nguyen Duc Chung (2021) Characteristic and amylase activity of yeasts isolated from banh men la starter tablets in central Vietnam. Plant Cell Biotechnology and Molecular Biology, 22(65-66), 69-80.
18. Le Thi Kim Thoa, Trinh Thi Phuong Thao, Nguyen Bao Hung, Kuan Shiong Khoo, Hoang Tan Quang, Tran Thuy Lan, Vu Duc Hoang, Seung-Moon Park, Chien Wei Ooi, Pau Loke Show, Nguyen Duc Huy (2022) Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase Expressed from Fusarium oxysporum. Waste and Biomass Valorization.
19. Nguyen Thi Thu Lien, Le Thi Tuyet Nhan, Ho Nhat Quang, Phan Thi Thao Nguyen, Truong Thi Bich Phuong, Dang Thi Thanh Loc, Le Van Tuan, Hoang Tan Quang (2022) The plant growth promotor auxin (IAA) production ability in Arthrospira massartii – ARH strain isolated from Vietnam and its potential applications. Plant Cell Biotechnology and Molecular Biology 23(1&2): 101-110.
20. Nguyen Thanh Tung, Pham Thi Diem Thi, Dang Ngoc Sang, Do Thi Thao, Hoang Tan Quang (2022) Biomass accumulation of Gynostemma pentaphyllum (Thunb.) Makino in cell suspension cultures inhibiting human cancer cell growth. Research Journal of Biotechnology 17(3): 61-68.
21. Hoang Tan Quang, Pham Thi Diem Thi, Dang Ngoc Sang, Tran Thi Ngoc Tram, Nguyen Duc Huy, Tran Quoc Dung, Quach Thi Thu The (2022) Effects of plant elicitors on growth and gypenosides biosynthesis in cell culture of Giao co lam (Gynostemma pentaphyllum). Molecules 27(9): 2972.
22. Dang Thanh Long, Hoang Tan Quang, Nguyen Thi Quynh Trang, Hoang Ngoc Han (2022) Study on the isolation and bioactivity assay of some compounds from Nelumbo nucifera Gaertn seeds collected at Tinh Tam lake, Thua Thien Hue, Vietnam. Plant Cell Biotechnology and Molecular Biology, 23(21-22): 42-53.
23. Tran Van Chen, Nguyen Duc Tuan, Nguyen Thanh Triet, Nguyen Hoang An, Phan Thi Thao Nguyen, Nguyen Thi Thanh Hai, Nguyen Thanh To Nhi, Nguyen Quang Co, Ho Thi Hoang Nhi, Hoang Viet Huong, Truong Thi Bich Phuong, Nguyen Thi Ai Nhung (2022) Morphological and molecular characterization of Distichochlamys citrea M.F. Newman in Bach Ma National Park, Thua Thien Hue Province, Vietnam. Biodiversitas 23: 2066-2079.
24. Xuan T. T. Phan, Binh V. Ngo, Huy D. Nguyen, Quang T. Hoang, Chung D. Ngo, Chinh T. Bui, Ty Nguyen, Nghiep T. Hoang (2022) Evaluating and reconstructing the genetic diversity of butterfly lizards of the genus Leiolepis Cuvier, 1829 (Reptilia: Squamata: Agamidae) in central Vietnam. Russian Journal of Herpetology (accepted)
25. Anh Tuan Nguyen, Hoc Tan Dao, Hoang Tan Quang, Seishi Hagihara, Mark Lokman, Erin Damsteegt (2022) Genetic diversity and population structure of the giant mottled eel, Anguilla marmorata in Central Vietnam. Fishes (accepted)

VII. SCIENTIFIC AWARDS

1. Third award from Thua Thien Hue contest on Science and Technology, 2018 

Title: Identification manganese peroxidase production fungi from Thua Thien Hue

Author: Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Le Thi Dieu Huyen, Nguyen Thi Thanh Tuyen, Nguyen Thi Be

2. 2021 Co Do award in science and technology

Title: Research on conservation and development of Curculigo orchioides Gaertn in mid – central Vietnam

Author: Pham Thanh, Tran Minh Duc, Nguyen Hoi, Tran Van Giang, Nguyen Ty, Bui Van Loi, Hoang Tan Quang, Vu Thi Quynh Trang, Huynh Thi Ngoc Diep, Bui Le Thanh Nhan, Nguyen Thanh Hung, Tran Quoc Dung, Nguyen Ngoc Hoa