Isolation and screening of Vibrio parahaemolyticus strains to cause acute hepatopancreatic necrosis disease in white-leg shrimps cultured in Phong Dien, Thua Thien Hue using 16S rRNA marker
Summary: In recent years, acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei has been one of the diseases that cause serious damage to the shrimp farming industry in Phong Dien district, Thua Thien Hue province. The disease causes mass death of shrimps at the age of 20–45 days (mortality rate up to 100%) in both Penaeus monodon and Litopenaeus vannamei. The agent causing AHPND is Vibrio spp. strains containing two PirA and PirB toxin genes on the same plasmid. Using the PCR technique with two specific primers, we identified two strains of Vibrio parahaemolyticus, namely K5 and K21 bearing PirA and PirB toxin genes from five isolates. The results of the sequence analysis of 16S rRNA gene confirmed that Vibrio sp. K5 and Vibrio sp. K21 belong to Vibrio parahaemolyticus.
Type: Domestic
Author: Nguyễn Văn Khanh, Nguyễn Quang Linh, Trần Thúy Lan, Lê Thị Tuyết Nhân, Nguyễn Thị Kim Cơ, Trần Quốc Dung
Unit: Laboratory of Protein and Enzyme technology, Laboratory of Gene technology, Laboratory of Cell technology
Journal:Hue University Journal of Science: Natural Science
Issue, Number, Pages128, 1E, 47-58
ISSN/ISBN:pISSN 1859–1388; eISSN 2615–9678
Impact factor:
Score according to the decision of the National Council for Professor in Academic statue:0,75
Year of publication: 2019
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