LABORATORY OF PROTEIN AND ENZYME TECHNOLOGY

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I. STAFFS

Dr. Nguyen Duc Huy
Head of laboratory
Phone: +842343813988
Email: ndhuy@hueuni.edu.vn

MSc. Le My Tieu Ngoc
Researcher
Phone: +8493.506.81.11
Email: lmtngoc@hueuni.edu.vn

BSc. Do Tran Huong Duyen
Researcher
Phone: +8491.35.88.183

II. RESEARCH INTERESTS

  • Isolation and identification high enzyme production microorganisms.
  • Application on using enzyme from microorganism for treatment environment pollution, aquaculture, enhance food storage and processing, and produce bio-fertilizer.
  • Production and application of recombinant microbial enzymes.
  • Identification and qualification metabolism of production pathway of enzyme from microorganism.
  • Enzyme structure, modeling and characterization.
  • Enzyme immobilization and bio-functional membrane
  • Optimization fermentation technology and processing for enzyme and protein production.

III. PROJECTS

  • Hue University (2015-2017): Identification of manganese peroxidase fungi isolated in Thua Thien Hue. PI: Nguyễn Đức Huy.
  • International (2016-2017): Peroxidases production from wood decay fungi using rice straw medium. PI: Nguyen Duc Huy.
  • International (2017-2018): Detoxification of antibiotics commonly using in aquaculture by Fusarium oxysporum. PI: Nguyen Duc Huy.
  • Hue University (2018-2019): Nghiên cứu tạo dòng và phân tích trình tự gen laccase từ Fusarium oxysporum HUIB02. PI: Nguyen Duc Huy.
  • International (2018-2019): Production of recombinant laccase and application on synthesis dyes degradation. Chủ nhiệm: PI: Nguyen Duc Huy.

IV. PUBLICATIONS

International journal

  1. Nguyen Duc Huy, Thayumanavan Palvannan, Tae-Ho Kwon and Seung-Moon Park. 2013. Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium. Journal of Bioscience and Bioengineering. 116(2):152-9.
  2. Nguyen Duc Huy, Cu Le Nguyen, Jeong-Woo Seo, Dae-Hyuk Kim, Seung-Moon Park. 2015. Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase. Journal of Bioscience and Bioengineering. 119(4): 416–420.
  3. Nguyen Hoang Loc, Le My Tieu Ngoc, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Ngoc Luong. 2015. Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris. Chemical Papers. 70(3): 284–293.
  4. Nguyen Duc Huy, Cu Le Nguyen, Nguyen Hoang Loc, Myoung-Suk Choi, Dae-Hyuk Kim, Jeong-Woo Seo, and Seung-Moon Park (2016). Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium. Journal of Bioscience and Bioengineering. 121(2): 154-159.
  5. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy. 2016. Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (Centella asiatica (L.) Urban) cells. 3 Biotech. 6(1): 86
  6. Nguyen Thanh Giang, Nguyen Duc Huy, Le The Ha Thanh, Nguyen Hoang Loc. 2016. Efficiency of the combination of salicylic acid, yeast extract and methyl jasmonate on asiaticoside production in centella (Centella asiatica (L.) urban) cells. Plant Cell Biotechnology and Molecular Biology. 17(1-2): 64-71.
  7. Hoang Tan Quang, Cai Thi Quynh Thoa, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Bich Phuong and Van Thi Yen. 2016. Study on genetic diversity of Paris polyphylla population from Vietnam and China. Plant Cell Biotechnology and Molecular Biology. 17(1-2): 57-63.
  8. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Phuong Lan. 2017. Accumulation of madecassoside – a major component of centelloside – in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid. Periodicum Biologorum. 119(1): 81-85.
  9. Nguyen Duc Huy, Nguyen Thi Thanh Tien, Le Thi Huyen, Hoang Tan Quang, Truong Quy Tung, Nguyen Ngoc Luong, Seung-Moon Park. 2017. Screening and production of manganese peroxidase from Fusarium on residue materials. Mycobiology. 45(1): 52-56.
  10. Nguyen Thi Anh Thu, Ngo Thi Tuong Chau, Le Van Thien, Nguyen Duc Huy, Nguyen Tran Me Khue, Nguyen Bao Hung, Nguyen Ngoc Luong, Le Thi Anh Thu, Nguyen Hoang Loc. 2018. Extra-chromosomal expression of nat05 gene encoding an alkaline serine protease from Bacillus subtilis Biotechnologia (In press).
  11. Truong Thi Phuong Lan, Nguyen Duc Huy, Nguyen Ngoc Luong, Nguyen Van Nghi, Trinh Huu Tan, Le Viet Quan, Nguyen Hoang Loc 2018. Identification and characterization of genes in curcuminoid pathway of Curcuma zedoaria Current Pharmaceutical Biotechnology. DOI: 10.2174/1389201019666181008112244
  12. Nguyen Thi Anh Thu, Ngo Thi Tuong Chau, Le Van Thien, Nguyen Duc Huy, Nguyen Tran Me Khue, Nguyen Bao Hung, Nguyen Ngoc Luong, Le Thi Anh Thu, Nguyen Hoang Loc. 2018. Extra-chromosomal expression of nat05 gene encoding an alkaline serine protease from Bacillus subtilis N05. Biotechnologia. 99(4): 365–373.

Domestic journal

  1. Hoang Tan Quang, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Tran Minh Duc, Le My Tieu Ngoc. 2015. Initial study on on in vitro culture of Paris polyphylla Smith for saponin production. Pham Van Dong University Journal of Science and Technology. 7: 5-11.
  2. Hoang Tan Quang, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Phuong Lan. 2016. Effects of methyl jasmonate on saponin accumulation in cultured suspension cells of Paris polyphylla Proceeding of the 2nd national conference on biological research and teaching in Vietnam. 1176-1183.
  3. Nguyen Thanh Giang, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Huu Thuan Anh, Truong Thi Phuong Lan, Nguyen Hoang Loc. 2016. Effect of elicitor on asiaticoside accumulation in Centella (Centella asiatica (L.) urban) cells. Vietnam National University Journal of Science. 31(4S): 44-49.
  4. Dang Thi Thanh Ha, Le Kim Tuan, Dinh Thi Kim Thien, Pham Thi Ngoc Lan, Hoang Tan Quang, Tran Thuy Lan, Nguyen Duc Huy. Cloning and sequencing laccase 3 (Folac3) from Fusarium oxysporum. Hue University Journal of Science. 127(1C): 1-10.
  5. Duong Duc Hoang Sinh, Tran Vu Ngoc Thi, Le Thi Ha Thanh, Pham Thi Ngoc Lan, Nguyen Hoang Loc, Nguyen Duc Huy. 2018. Isolation of some bacterial strains from dioxin-contaminated soil in A luoi, Thua Thien Hue. Hue University Journal of Science. 127(1C): 141–148.

V. SCIENTIFIC AWARDS

1. 2017 Co Do award in science and technology

  • Title: Development and application of recombinant microbial enzymes.
  • Author: Nguyen Hoang Loc, Do Thi Bich Thuy, Tran Thi Thu Ha, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Bao Hung, Nguyen Thi Quy Hoa, Le My Tieu Ngoc, Nguyen Ngoc Luong.

2. Third award from ThuaThienHue contest on Science and Technology, 2018 

  • Title: Identification manganese peroxidase production fungi from Thua Thien Hue.
  • Author: Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Le Thi Dieu Huyen, Nguyen Thi Thanh Tuyen, Nguyen Thi Be.
English