LABORATORY OF ENZYME AND PROTEIN TECHNOLOGY

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I. Staffs

Nguyen Duc Huy, PhD

Head of laboratory

Phone: 02343813988

Email: ndhuy@hueuni.edu.vn

Le My Tieu Ngoc, MSc.

Researcher

Phone: 01676749484

Email: lemytieungoc@gmail.com

Nguyen Bao Hung, PhD

Researcher

Phone: 0906545902

Email: nguyenbaohung1986@gmail.com

 

Nguyen Van Khanh. MSc.

Researcher

Phone: 0396922302

Email: nvkhanh@hueuni.edu.vn

II. Research interests

– Isolation and identification high enzyme production microorganisms.

– Application on using enzyme from microorganism for treatment environment pollution, aquaculture, enhance food storage and processing, and produce bio-fertilizer.

– Production and application of recombinant microbial enzymes.

– Identification and qualification metabolism of production pathway of enzyme from microorganism

– Enzyme structure, modeling and characterization.

– Enzyme immobilization and bio-functional membrane.

– Optimization fermentation technology and processing for enzyme and protein production.

– Knock out technology for bacterial and study the gene function.

– Microbial safety analysis on food and vegetable and GAP guideline for agricultural application.

III. Projects

– Hue University (2015-2017): Identification of manganese peroxidase fungi isolated in Thua Thien Hue. PI: Nguyen Duc Huy

– Hue University (2015-2017): Building the Cultivation and processing procedures of Spirulina platensis as supplementary food. PI: MSc. Nguyen Van Khanh.

– International (2016-2017): Peroxidases production from wood decay fungi using rice straw medium. PI: Nguyen Duc Huy

– International (2017-2018): Detoxification of antibiotics commonly using in aquaculture by Fusarium oxysporum. PI: Nguyen Duc Huy

– Hue University (2018-2019): Nghiên cứu tạo dòng và phân tích trình tự gen laccase từ Fusarium oxysporum HUIB02. PI: Nguyen Duc Huy

– Hue University (2018-2019): Identification of genes causing acute hepatopancreatic necrosis disease in white-leg shrimp from Vibrio isolated in Phong Dien District, Thua Thien Hue Province. PI: MSc. Nguyen Van Khanh.

– International (2018-2019): Production of recombinant laccase and application on synthesis dyes degradation. PI: Nguyen Duc Huy

– Ministry of Education and Training (2018-2020): Development microorganism solution against Vibrio sp. for prevention and treatment diseases on shrimp, fishes. PI: Nguyen Duc Huy

– Ministry of Education and Training (2018-2020): Antibody production for prevention and treatment Vibrio spp. causing Acute Hepatopancreatic Necrosis Disease in white-leg shrimp (Litopenaeus vannamei). PI: MSc. Nguyen Van Khanh.

IV. Publications

International journal

  1. Nguyen Duc Huy, Thayumanavan Palvannan, Tae-Ho Kwon and Seung-Moon Park. 2013. Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium. Journal of Bioscience and Bioengineering. 116(2):152-9.
  2. Nguyen Duc Huy, Cu Le Nguyen, Jeong-Woo Seo, Dae-Hyuk Kim, Seung-Moon Park. 2015. Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a β-xylosidase that cleaves xylans in synergistic action with endo-xylanase. Journal of Bioscience and Bioengineering. 119(4): 416–420.
  3. Nguyen Hoang Loc, Le My Tieu Ngoc, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Ngoc Luong. 2015. Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris. Chemical Papers. 70(3): 284–293.
  4. Nguyen Duc Huy, Cu Le Nguyen, Nguyen Hoang Loc, Myoung-Suk Choi, Dae-Hyuk Kim, Jeong-Woo Seo, and Seung-Moon Park (2016). Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium. Journal of Bioscience and Bioengineering. 121(2): 154-159.
  5. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy. 2016. Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (Centella asiatica (L.) Urban) cells. 3 Biotech. 6(1): 86
  6. Nguyen Thanh Giang, Nguyen Duc Huy, Le The Ha Thanh, Nguyen Hoang Loc. 2016. Efficiency of the combination of salicylic acid, yeast extract and methyl jasmonate on asiaticoside production in centella (Centella asiatica (L.) urban) cells. Plant Cell Biotechnology and Molecular Biology. 17(1-2): 64-71.
  7. Hoang Tan Quang, Cai Thi Quynh Thoa, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Bich Phuong and Van Thi Yen. 2016. Study on genetic diversity of Paris polyphylla population from Vietnam and China. Plant Cell Biotechnology and Molecular Biology. 17(1-2): 57-63.
  8. Nguyen Hoang Loc, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Phuong Lan. 2017. Accumulation of madecassoside – a major component of centelloside – in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid. Periodicum Biologorum. 119(1): 81-85.
  9. Nguyen Duc Huy, Nguyen Thi Thanh Tien, Le Thi Huyen, Hoang Tan Quang, Truong Quy Tung, Nguyen Ngoc Luong, Seung-Moon Park. 2017. Screening and production of manganese peroxidase from Fusarium on residue materials. Mycobiology. 45(1): 52-56.
  10. Truong Thi Phuong Lan, Nguyen Duc Huy, Nguyen Ngoc Luong, Nguyen Van Nghi, Trinh Huu Tan, Le Viet Quan, Nguyen Hoang Loc 2018. Identification and characterization of genes in curcuminoid pathway of Curcuma zedoaria Current Pharmaceutical Biotechnology. DOI: 10.2174/1389201019666181008112244
  11. Nguyen Thi Anh Thu, Ngo Thi Tuong Chau, Le Van Thien, Nguyen Duc Huy, Nguyen Tran Me Khue, Nguyen Bao Hung, Nguyen Ngoc Luong, Le Thi Anh Thu, Nguyen Hoang Loc. 2018. Extra-chromosomal expression of nat05 gene encoding an alkaline serine protease from Bacillus subtilis N05. Biotechnologia. 99(4): 365–373.
  12. Bohyun Yun, HM An, Won-Bo Shim, Won-Il Kim, Nguyen Bao Hung, S Han, Hyun Ju Kim, Seung don Lee, Se-Ri Kim. Development of a Screening Method and Device for the Detection of Escherichia coli from Agri-Food Production Environments and Fresh Produce. 2017.J MicrobiolBiotechnol.27(12):2141-2150
  13. Dhinesh Kumar Rajendran, Eunsoo Park, Rajalingam Nagendran, Nguyen Bao Hung, Byoung-Kwan Cho, Kyung-Hwan Kim, Yong Hoon Lee. Visual analysis for detection and quantification of Pseudomonas cichorii disease severity in tomato plants. 2016. Plant Pathology J. 7.
  14. Bohyun Yun, HM An, Won-Bo Shim, Won-Il Kim, Nguyen Bao Hung, S Han, Hyun Ju Kim, Seung don Lee, Se-Ri Kim. Development of a Screening Method and Device for the Detection of Escherichia coli from Agri-Food Production Environments and Fresh Produce. 2017.J MicrobiolBiotechnol.27(12):2141-2150
  15. Nguyen Bao Hung, Bohyun Yun, Won-Il Kim, Gyusuck Jung, Theresa Lee, Eunjung Roh, Hyun Ju Kim, Seungdon Lee, Se-Ri Kim. Analysis of the microbial contamination levels in dried red pepper during production. 2018. Korean J. Food Preserv. 25(2): 279-287
  16. Nguyen Bao Hung, Hyeonjin Chu, Won-Il Kim, Injun Hwang, Hyun-Ju Kim, Hwangyong Kim, Kyoungyul Ryu, Se-Ri Kim. 2019. Development of device based on IoT technology for detecting Escherichia coli from various agri-foods and production environments. Korean J. Food Preserv.
  17. Se-ri Kim, Hyeonjin Chu, Seung-Won Yi, Youn-Jung Jang, Nguyen Bao Hung, Won-Il Kim, Hyun-Ju Kim, Kyoungyul Ryu. 2019. Investigation of microbial contamination level during production of dried red pepper. Korean J. Food Preserv.
  18. Nguyen Van Khanh, Le Thi Tuyet Nhan, Nguyen Thi Diem, Phan Van Cu, Tran Quang Khanh Van, Ngo Thi Hoan (2017). The Effects of Nutritional Media and Initial Cell Density on the Growth and Development of Spirulina platensis. Journal of Agriculture Science and Technology A and B & Hue University Journal of Science. 07:60-67. DOI:10.17265/2161-6256/2017.10.008S.
  19. Tran Vinh Phuong, Nguyen Van Khanh, Pham Thi Hai Yen, Vo Dieu, Nguyen Van Huy (2017). Effects of Foods on Maturity and Spawning Induction Methods on Ovulation of Rice Field Eel Monopterus albus (Zuiew, 1793) in Thua Thien Hue Province, Vietnam. Journal of Agriculture Science and Technology A and B & Hue University Journal of Science. 07:85-92. DOI:10.17265/2161-6256/2017.10.011S.
  20. Nguyen Vinh Truong, Tran Thi Bach Thao, Tran Thi Anh Tuyet, Nguyen Van Khanh and Tran Quang Khanh Van (2017). Safe Vegetable Consumption in Hue City: A Case Study of Food Safety Situation in VietNam. Journal of Agriculture Science and Technology A and B & Hue University Journal of Science. 07:119-130. DOI: 10.17265/2161-6256/2017.10.015S

Domestic journal

  1. Hoang Tan Quang, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Tran Minh Duc, Le My Tieu Ngoc. 2015. Initial study on on in vitro culture of Paris polyphylla Smith for saponin production. Pham Van Dong University Journal of Science and Technology. 7: 5-11.
  2. Hoang Tan Quang, Trinh Huu Tan, Nguyen Thanh Giang, Nguyen Duc Huy, Truong Thi Phuong Lan. 2016. Effects of methyl jasmonate on saponin accumulation in cultured suspension cells of Paris polyphylla Proceeding of the 2nd national conference on biological research and teaching in Vietnam. 1176-1183.
  3. Nguyen Thanh Giang, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Huu Thuan Anh, Truong Thi Phuong Lan, Nguyen Hoang Loc. 2016. Effect of elicitor on asiaticoside accumulation in Centella (Centella asiatica (L.) urban) cells. Vietnam National University Journal of Science. 31(4S): 44-49.
  4. Dang Thi Thanh Ha, Le Kim Tuan, Dinh Thi Kim Thien, Pham Thi Ngoc Lan, Hoang Tan Quang, Tran Thuy Lan, Nguyen Duc Huy. Cloning and sequencing laccase 3 (Folac3) from Fusarium oxysporum. Hue University Journal of Science. 127(1C): 1-10.
  5. Duong Duc Hoang Sinh, Tran Vu Ngoc Thi, Le Thi Ha Thanh, Pham Thi Ngoc Lan, Nguyen Hoang Loc, Nguyen Duc Huy. 2018. Isolation of some bacterial strains from dioxin-contaminated soil in A luoi, Thua Thien Hue. Hue University Journal of Science. 127(1C): 141–148.
  6. Nguyen Van Khanh*, Nguyen Quang Linh, Tran Thuy Lan, Le Thi Tuyet Nhan, Tran Quang Khanh Van, Nguyen Thi Kim Co, Tran Quoc Dung (2019). Isolation and screening of Vibrio parahaemolyticus strains causing Acute Hepatopancreatic Necrosis Disease in cultured whiteleg shrimp in Phong Dien district, Thua Thien Hue province by 16S rRNA marker. Hue University Journal of Science: Natural science, 128(1E), pp 5-10. (Vietnamese).
  7. Nguyen Van Khanh, Phan Van Cu (2019). Antibacterial activity of silver nanoparticles against Aeromonas and Vibrio spp. isolated from aquaculture water environment in Thua Thien Hue. Hue University Journal of Science: Agriculture and Rural Development, 128(3B). pp 5–16. DOI: 10.26459/hueuni-jard.v128i3B.4615 (English).
  8. Nguyen Van Hue, Nguyen Duc Huy, Nguyen Van Khanh and Nguyen Quang Lich (2019). Influence of nutrition medium and seasonal variation on mushroom (Volvariella volvacea) propagation phases. Hue University Journal of Science: Agriculture and Rural Development, 129(3B). pp 5–18. DOI: 10.26459/hueuni-jard.v128i3D.53995 (Vietnamese).
  9. Tran Vinh Phuong, Nguyen Van Khanh, Pham Thi Hai Yen, Kieu Thi Huyen, Vo Duc Nghia, Le Thi Tuyet Nhan (2017). Effects of nutrient environment, initial density on growth of two species of Chaetoceros muelleri, Tetraselmis suecica and experimental on biomass culture in natural light conditions in Thua Thien Hue. Hue University Journal of Science: Agriculture and Rural Development, 126 (3D): 119-129. DOI: http://dx.doi.org/10.26459/hueuni-jard.v126i3D.4300.
  10. Le Nhu Phuong, Nguyen Van Khanh (2015). Biological characteristics and potential development of Terapon jarbua in the Tam Giang – Cau Hai lagoon. Journal of Science – Hue University. Hue University Journal of Science: Agriculture and Rural Development, 104(05): 189-205 (Vietnamese).
  11. Kieu Thi Huyen, Nguyen Van Khanh, Tran Nguyen Ngoc (2015). Analysis of economic efficiency and factors affecting profits of polyculture models Penaeus monodon with fish in Tam Giang – Cau Hai lagoon. Hue University Journal of Science: Agriculture and Rural Development. 04/2015: 167-174. (Vietnamese).
  12. Kieu Thi Huyen, Nguyen Van Khanh (2015). Assessment of adaptability to climate change of the polyculture models between Penaeus monodon, Mugil cephalus, Siganus canaliculatus and Siganus guttatus in Tam Giang – Cau Hai lagoon, Thua Thien Hue province. Hue University Journal of Science: Agriculture and Rural Development, 104(05):107-118 (Vietnamese)
  13. Ngo Huu Toan, Nguyen Van Khanh (2014). Effect of salinity to the speed of growth and survival rate of anabas testudineus bloch, 1792. Journal of Agriculture and Rural Development, 4/2014: 137-143 (Vietnamese)
  14. Ngo Huu Toan, Nguyen Van Khanh (2013). Adaptability to salinity of square-head climbing perch (Anabas testudineus Bloch, 1792). Journal of Agriculture and Rural Development, 24/2013: 87-91 (Vietnamese).
  15. Ho Thi Tung, Tran Quang Khanh Van, Nguyen Van Khanh (2014). Inhibition abilities of Bacillus spp. to Vibrio parahaemolyticus on early mortality syndrome in Thua Thien Hue. Hue University Journal of Science: Agriculture and Rural Development, 98 (10): 147-160. (English).
  16. Thi Bich Dao, Nguyen Van Khanh, Nguyen Quang Linh (2014). Research some of characteristics of Vibrio parahaemolyticus which causes EMS disease on juvenile shrimp at Dien Huong community, Phong Dien district, Thua Thien Hue province. Hue University Journal of Science: Agriculture and Rural Development, 98 (10): 13-22. (English)
  17. Nguyen Thi Bich Dao, Tran Quang Khanh Van, Nguyen Van Khanh, Nguyen Quang Linh (2014). Isolation and selection bacillus strains to inhibit vibrio parahaemolyticus which is the cause of ems on shrimp farming in Thua Thien Hue province, Viet Nam. Hue University Journal of Science: Agriculture and Rural Developmen, 100(01):15-28 (Vietnamese).

V. Scientific awards

1. 2017 Co Do award in science and technology

Title: Development and application of recombinant microbial enzymes.

Author: Nguyen Hoang Loc, Do Thi Bich Thuy, Tran Thi Thu Ha, Hoang Tan Quang, Nguyen Duc Huy, Nguyen Bao Hung, Nguyen Thi Quy Hoa, Le My Tieu Ngoc, Nguyen Ngoc Luong

2. Third award from ThuaThienHue contest on Science and Technology, 2018

Title: Identification manganese peroxidase production fungi from Thua Thien Hue

Author: Nguyen Duc Huy, Hoang Tan Quang, Tran Thuy Lan, Le Thi Dieu Huyen, Nguyen Thi

3. Consolation prize in Thua Thien Hue Science Technology Creation Award in 2018.

Title: Building the Cultivation and processing procedures of Spirulina platensis as supplementary food.

Authors: Nguyen Van Khanh, Phan Van Cu, Nguyen Thi Diem, Le Thi Tuyet Nhan

4. Excellent poster awards: 2018/11. 33thInternational symposium and Annual meeting, Korean Society of Food Science and Technology (KoSFoST), Korea. (Nguyen Bao Hung)

5. Excellent research team of National Institute of Agricultural Sciences (2018) (Nguyen Bao Hung)

6. Excellent research team of National Institute of Agricultural Sciences (2019) (Nguyen Bao Hung)

7. Third prize in Thua Thien Hue Science and Technology Innovation Award 2019.

Title: Technological process for producing Monopterus albus (Zuiew, 1793) by semi-natural method.

Authors: Tran Vinh Phuong, Nguyen Van Khanh, Pham Thi Hai Yen, Vo Dieu, Nguyen Thi Diem

VI. Technology transfer products

 

 

 

 

 

 

 

English