Molecular Markers Linked to Phytophthora Root Rot Resistancein Pepper (Capsicum annuum L.)
Summary: Phytopthora root rot in pepper (C. annuum) is caused by Phytophthora capsici L. which exhibits a high level of pathogenic diversity. Resistance to this disease is conditioned by number of quantitative trait loci. Pyramiding resistance alleles is desirable and could be simplified by the use of molecular markers tightly linked to the resistance. The purpose of this study was the development of molecular markers linked to the Phytophthora root rot resistance. An F8 RIL population derived from across between YCM334 and a susceptible cultivar ‘Tean’ was used in combination with bulk segregation analysis utilizing the random amplified polymorphism DNA (RAPD) and conversion of amplified fragment length polymorphism (AFLP) markers linked to Phytophtora root rot resistance into sequence-characterized amplified region (SCAR) markers. In conversion of the three AFLP markers, only one was successfully converted into a co-dominant SCAR marker SA133_4 linking to the trait. In bulked segregant analysis (BSA), out of 400 random amplified polymorphic DNA (RAPD) primers, only three (UBC484, 504, and 553) produced polymorphic RAPD markers between the two DNA pools. Genetic linkage analysis showed that the SCAR and RAPD markers located in the chromosome 5 of pepper in a reference map. QTL analysis showed that SA133_4 and UBC553 linked to Phytophtora root rot resistance. These markers were correctly identified as resistant or susceptible of nine commercial pepper varieties. The obtained markers SA133_4 and UBC553 will be beneficial to marker-assisted selection in pepper breeding
Type: Non-ISI/SCOPUS listed journals with ISSB/ISBN
Author: Trương Thị Hồng Hải, JH. Kim, MC. Cho, SY. Chae
Unit: Director board of Institute
Journal:Korean Journal Horticulture Science Technology
Issue, Number, Pages30, Supply I, 93
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Year of publication: 2012
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