In vivo Evaluation of Transgenic Watercress Containing Gene Encoding Escherichia coli Heat-Labile Toxin B Subunit
Summary: In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg g-1), photosynthetic rate (23.39 mgCO2 [dm2]-1 h-1), dry matter (7.42%), vitamin C (0.34 mg g-1), calcium (0.83 mg g-1), and potassium (2.47 mg g-1). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1-2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46% of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors.
Type: SCOPUS listed journals
Author: Nguyễn Hoàng Lộc, Nguyễn Văn Song, Đặng Thanh Long, Tae-Geum Kim, Moon-Sik Yang
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Journal:Journal of Plant Biochemistry and Biotechnology (0971-7811, E-ISSN: 0974-1275)
Issue, Number, Pages24, 2, 129-134
ISSN/ISBN:0971-7811, E-ISSN: 0974-1275
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Year of publication: 2015
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