Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (Centella asiatica (L.) Urban)cells
Summary: In this study, we report the expression level of CaSQS, CabAS and CaCYS, the genes involved in phytosterol and triterpene metabolic pathway of centella (Centella asiatica (L.) Urban), in cells elicited with salicylic acid (50–200 lM). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis indicated CaSQS, CabAS, and CaCYS genes expressed in both the wild-type and cultured cells (with and without elicitation). In elicited cells, expressions of CaSQS, CabAS, and CaCYS genes showed strong dependence on salicylic acid concentration and elicitation day. The highest expression of CabAS gene was found in the cells elicited with 100 lM salicylic acid on day 10 of inoculation. Salicylic acid treatment (50–200 lM) decreased expression level of CaCYS and CaSQS genes in elicited cells compared with the control.
Type: SCOPUS listed journals
Author: Nguyen Hoang Loc, Nguyen Duc Huy, Nguyen Thanh Giang
Unit: Director board of Institute
Journal:3 Biotech
Issue, Number, Pages6, 86, 1-7
ISSN/ISBN:2190 - 572X, E-ISSN 2190 - 5738
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Year of publication: 2016
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