Genetic variability of “Thanh tra” pummelo (Citrus grandis (L.) Osbeck) at Thua Thien Hue, Vietnam
Summary: “Thanh tra” pummelo (Citrus grandis (L.) Osbeck) is a kind of speciality fruit tree in Thua Thien Hue province, Vietnam, they have significant differences in most of tree and fruit morphology characteristics such as tree and leaf size, fruit shape and size. The genetic variability of 42 “Thanh tra” pummelo trees was evaluated using random amplified polymorphic DNA (RAPD) analyses. All of the 68 amplified fragments revealed polymorphism among the trees, with the average number of polymorphic markers per primer being 7.56. The number of amplified fragments per primer varied from 5 to 11, with their sizes ranging from approximately 305 to 2,354 bp. Jaccard’s similarity coefficient values changed from 0.00 to 1.00 with a mean of 0.26. An unweighted pair group method with arithmetic (UPGMA) phenetic tree was constructed and three “Thanh tra” pummelo were identified. Group A contains 31 trees with Jaccard’s coefficients of genetic similarity (GS) approximately of 0.23 and subdivided into four subgroups, most of all were planted at the south of the Duong Hoa and Khuc Ly. Group B consist of 5 trees from all of collected sample regions except Duong Hoa (8TB, 11TB, 11TB, 8PS, 5PS and 27KL). Group C includes 6 genotypes (2TB, 3TB, 8PA, 11KL, 13KL and 26KL), explants in this group have less than 10 PCR products and genetic similarity less than 50%. “Thanh tra” pummelos in Thua Thien Hue have high level of genetic diversity.
Type: International
Author: Hoang Tan Quang, Le Thi Xuan, Ngo Thi Mai, Nguyen Van Trung, Truong Thi Bich Phuong, Nguyen Hoang Loc, Nguyen Thi Thu Lien
Unit: Laboratory of Gene technology, Director board of Institute
Journal:Annals of Bological Research
Issue, Number, Pages2, 4, 306-314
ISSN/ISBN:0976-1233
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Year of publication: 2011
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