Genetic diversity of earthworm Amynthas rodericensis (Grube, 1879) (Clitellata: Megascolecidae) in Vietnam by randomly amplified polymorphic DNA analysis
Summary: In this study, Random Amplified Polymorphic DNA (RAPD) markers were applied to analyse the genetic diversity of samples of the Amynthas rodericensis (Grube, 1879), collected from six localities (viz., Hai Lang along Quang Tri province; Phong Dien, Hue, and Huong Thuy along Thua Thien Hue province in Vietnam). The eight primers used in RAPD analysis amplified 71 loci, all of which were polymorphic. The percentages of polymorphic loci observed in the four populations were: 71.83% (Hai Lang), 87.32% (Phong Dien), 73.24% (Hue), and 38.03% (Huong Thuy). Data for observed and effective number of alleles, Nei’s (1973) genetic diversity, and Shannon’s information index, for all the four populations were 1.6761, 1.4802, 0.2718 and 0.3971, respectively. The value for total genotype diversity among populations was 0.3631 while within populations was found to be 0.2718. The mean coefficient of gene differentiation value and the estimate of gene flow across the populations were found as 0.2515 and 1.4870, respectively. The Nei (1972) measures of genetic distance and identity between pairs of earthworm populations indicate that the population originated from Phong Dien and Hue has the highest genetic identity, while the earthworms originated from Hai Lang and Huong Thuy show the greatest genetic distance.
Type: International
Author: Nguyễn Văn Thuận, Trần Văn Giang, Nguyễn Trần Trung, Hoàng Tấn Quảng, Trần Quốc Dung
Unit: Laboratory of Gene technology
Journal:Journal of Chemical, Biological and Physical Sciences An International Peer Review E-3 (2249 –1929)
Issue, Number, Pages8, 4, 870-883
ISSN/ISBN:2249-1929
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Year of publication: 2018
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