Comparative Mapping of Consensus SSR Markers in an Intraspecific F8 Recombinant Inbred Line Population in Capsicum
Summary: A saturated intraspecific genetic map is critical for studying QTLs associated with Phytophthora root rot resistance in pepper. The map was constructed using a population of 126 F8 recombinant inbred lines derived from a cross between YCM334 (resistant to Phytophthora root rot) and the susceptible local variety, Tean. To identify a set of consensus markers for mapping, 67 anchor SSR markers were selected from the reference map Pepper-FAO3 and 130 from SNU3. Polymorphic rates were low: 43 out of 197 were polymorphic. In addition, 1,667 EST-SSR primers were used. Given 11% of polymorphism rate was enough to frame, but not to saturate the map. To saturate the map, 66 AFLP primer combinations were also used. Among the 454 markers used, 281 AFLPs, 101 EST-SSRs, 37 consensus SSRs and 1 CAPS marker were mapped and distributed in 19 linkage groups (LGs). Based on distribution of the consensus markers, 14 linkage groups were assigned into 12 chromosomes of pepper. The map covered 2177.5 cM with an average of 5.2 cM. Distribution and order of consensus markers in the present linkage map were consistent with the previously developed maps. The map will become a useful tool for analyzing QTLs of the mapping population.
Type: ISI listed journals
Author: Trương Thị Hồng Hải, Ki-Taek Kim, Su Kim , Young Chae, Jeong-Hyun Park, Dae-Geun Oh, Myoung-Cheol Cho
Unit:
Journal:Horticulture, Environment, and Biotechnology
Issue, Number, Pages51, 3, 193-206
ISSN/ISBN: 2211-3460
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Year of publication: 2010
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